Liu, Longlong; Patnana, Pradeep Kumar; Xie, Xiaoqing; Frank, Daria; Nimmagadda, Subbaiah Chary; Rosemann, Annegret; Liebmann, Marie; Klotz, Luisa Hildegard; Opalka, Bertram; Khandanpour, Cyrus: High Metabolic Dependence on Oxidative Phosphorylation Drives Sensitivity to Metformin Treatment in MLL/AF9 Acute Myeloid Leukemia
Inhalt
- Materials and Methods
- Cell Culture
- Seahorse Extracellular Flux Analysis
- Retrovirus Production and Transduction
- MLL/AF9 Leukemia Mouse Models
- Mitochondrial Membrane Potential (MMP), Mitochondrial Number Detection, and Cells Sorting
- Metformin Treatment
- Immunoblot Analysis
- Mitochondrial DNA Quantification
- Statistical Analysis
- Results
- Metabolic Pathway Dependence Analysis Shows Upregulated OXPHOS in MLL/AF9 AML Cells
- MLL/AF9 Gene Expression Is Associated with Increased Mitochondrial Respiration
- MLL/AF9 AML Cells Are More Sensitive to Metformin Treatment
- Discussion
- Conclusions
- References