Valla, Sofía; Hassan, Nourhan; Luján Vitale, Daiana; Madanes, Daniela; Spinelli, Fiorella Mercedes; Teixeira, Felipe C. O. B.; Greve, Burkhard; Espinoza-Sánchez, Nancy Adriana; Cristina, Carolina; Alaniz, Laura; Götte, Martin: Syndecan-1 Depletion Has a Differential Impact on Hyaluronic Acid Metabolism and Tumor Cell Behavior in Luminal and Triple-Negative Breast Cancer Cell [...]
Inhalt
- Results
- Syndecan-1 siRNA Knockdown Has a Differential Effect on HA Metabolism and Binding in MCF-7 and MDA-MB-231 Breast Cancer Cells
- Sdc-1 Depletion Inhibits Breast Cancer Stem Cell Properties in A Complex Interplay of EMT Transcription Factors and Wnt Signaling That Is Partially Modulated by HA
- Sdc-1 KD Exerts Different Effects on Cell Cycle Progression and Apoptosis in MCF-7 and MDA-MB-231 Cells with HA-Dependent Modulation of BAD Expression
- Syndecan-1 KD Downregulates Angiogenic Factor and miR-10b Expression in Tumor Cells, While HA Addition Exerts Opposite Effects on IL-8 mRNA Levels in MCF-7 and MDA-MB-231 Cells
- A Differential Kinase Activation Signature Is Associated with Sdc-1 Depletion in MDA-MB-231 and MCF-7 Cells
- STRING Analysis Reveals That Sdc-1 Interacts with Various Protein Targets Associated with HA Metabolism, Stemness and Development, Adhesion, Angiogenesis, Growth, and Different Signaling Pathways
- Discussion
- Conclusions
- Materials and Methods
- Cell Culture
- siRNA Transfection
- HA Treatment
- Total RNA Extraction and cDNA Synthesis
- Quantitative Real-Time PCR
- Protein Extraction
- SDS-Page and Immunoblotting
- Flow Cytometry
- HA Binding Ability
- HA Release
- ELISA
- Hanging Drop Assay
- STRING Protein-Protein Interaction Analysis
- TNM and Kaplan–Meier Plots
- Statistical Analysis
- References