Detzner, Johanna; Krojnewski, Elisabeth; Pohlentz, Gottfried; Steil, Daniel; Humpf, Hans-Ulrich; Mellmann, Alexander; Karch, Helge; Müthing, Johannes: Shiga Toxin (Stx)-Binding Glycosphingolipids of Primary Human Renal Cortical Epithelial Cells (pHRCEpiCs) and Stx-Mediated Cytotoxicity
Inhalt
- Results
- Identification of Stx1a- and Stx2a-Binding Globo-Series Glycosphingolipids of pHRCEpiCs
- Structural Characterization of Sphingolipids from the Neutral GSL Fraction of pHRCEpiCs
- Lipid Composition of DRM and Non-DRM Fractions Obtained from pHRCEpiCs
- Mass Spectrometric Specification of the Stx Receptor GSLs Gb3Cer and Gb4Cer in DRM and Non-DRM Fractions Obtained from pHRCEpiCs
- Mass Spectrometric Specification of the Phospholipids and SM in DRM and Non-DRM Fractions Obtained from pHRCEpiCs
- Stx1a- and Stx2a-Mediated Cellular Damage of pHRCEpiCs
- Discussion
- Conclusions
- Materials and Methods
- Cultivation and Propagation of pHRCEpiCs
- Stx Cytotoxicity Assay
- Harvest of DRM and Non-DRM Fractions from Sucrose Density Gradients
- Isolation and Purification of GSLs from Total Cells
- Obtaining Phospholipid and GSL Preparations from DRM and Non-DRM Fractions
- Stx1a, Stx2a, Antibodies, and Lipid References
- Thin-Layer Chromatography, Lipid Staining, and Overlay Detection
- Mass Spectrometry of GSLs
- References