Bobe, Stefanie; Beckmann, Daniel; Klump, Dorothee Maria; Dierkes, Cathrin; Kirschnick, Nils; Redder, Esther; Bauer, Nadine; Schäfers, Michael; Erapaneedi, Raghu; Risse, Benjamin; van de Pavert, Serge A.; Kiefer, Friedemann: Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice
Inhalt
- Material and methods
- Mouse strains
- Ultramicroscopy
- Immunofluorescence whole mount staining
- Immunofluorescence staining of sections
- Optical clearing
- Portal vein injection
- Antibodies
- Quantitative analysis of the lymphatic network
- Results
- Identifying hepatic lymphatic vessels
- Lymphatic vessels of the liver were located in portal tracts
- Volumetric imaging of lymphatic hepatic vessels
- Lymph flow in the murine liver
- Development of lymphatic vessels in the murine liver
- VEGF-C and VEGFR-3
- Discussion
- Data availability statement
- Ethics statement
- Author contributions
- Funding
- Conflict of interest
- Publisher’s note
- Supplementary material
- References