Troschel, Fabian Martin; Minte, Annemarie; Ismail, Yahia Mahmoud; Kamal, Amr; Abdullah, Mahmoud Salah; Ahmed, Sarah Hamdy; Deffner, Marie; Kemper, Björn; Kiesel, Ludwig; Eich, Hans-Theodor; Eich, Hans Theodor; Eich, Hans Theodor; Eich, Hans Theodor; Ibrahim, Sherif; Götte, Martin; Greve, Burkhard: Knockdown of Musashi RNA Binding Proteins Decreases Radioresistance but Enhances Cell Motility and Invasion in Triple-Negative Breast Cancer
Inhalt
- Introduction
- Results
- MSI Protein mRNAs Show Strong Correlations with Each Other and Notch Pathway Elements in Triple-Negative Breast Cancer Samples
- MSI-1 and MSI-2 Small Interfering RNA (siRNA) Transfection Results in MSI-1 and MSI-2 Knockdown
- Knockdown of MSI-1 and MSI-2 Suppresses the Notch Pathway
- Breast Cancer Stem Cell Markers Are Downregulated after MSI Protein Knockdown
- Decreased MSI Protein Expression Results in Increased Apoptosis and Reduced Proliferation
- Low MSI Expression Leads to Decreased Radioresistance and Reduced Expression of DNA Repair-Related Proteins DNA-PKcs and EGFR
- MSI-Downregulated Cells Exhibit Increased Cell Motility and Invasion
- Discussion
- MSI Protein Knockdown Critically Downregulates Stem Cell Characteristics and Cell Cycle Progression While Increasing Apoptosis Subsequent to Notch Pathway Inactivation
- MSI Protein Knockdown Reduces Breast Cancer Radioresistance via Downregulation of EGFR and DNA-PKcs
- MSI Protein Knockdown Results in a Higher Cell Invasiveness and More Migration In Vivo, Possibly due to Downregulation of the LIF Receptor
- Materials and Methods
- Cell Line and Transfection
- qPCR
- Western Blot Analysis
- Flow Cytometry
- Cell Colony Formation
- Cell Cycle Progression
- H2AX
- Apoptosis
- Invasion
- Digital Holographic Microscopy
- Primary Tissue
- Statistical Analysis
- References