Arranz San Martín, Alba; Vogel, Jan; Wullich, Sandra C.; Quax, Wim J.; Fetzner, Susanne: Enzyme-Mediated Quenching of the Pseudomonas Quinolone Signal (PQS): A Comparison between Naturally Occurring and Engineered PQS-Cleaving Dioxygenases
Inhalt
- Materials and Methods
- Chemicals
- Bacterial Strains, HQD Enzymes, and Plasmids
- Heterologous Production and Purification of Dioxygenases
- Enzyme Assay
- Cultivation of P. aeruginosa in the Presence of Quorum Quenching Enzymes
- Determination of AQ Levels
- Quantification of Virulence Factors
- Galleria mellonella Infection Model
- Statistical Analysis
- Results
- Catalytic Half-Lives of PQS Dioxygenases
- PQS Dioxygenases in Contrast to HodC Are Not Inactivated by P. aeruginosa Exoproducts
- PQS Dioxygenases from N. farcinica and S. bingchenggensis Quench AQ and Virulence Factor Production
- PQS Dioxygenase from S. bingchenggensis, as Well as Small Molecule Inhibitors of AQ Biosynthesis, Enhance G. mellonella Survival upon Infection with P. aeruginosa PAO1
- Discussion
- References