Schmidt, Tobias; Ghaffarian, Nikoo ; Philippot, Camille; Seifert, Gerald ; Steinhäuser, Christian ; Pape, Hans-Christian ; Blaesse, Peter: Differential regulation of chloride homeostasis and GABAergic transmission in the thalamus
Inhalt
- Differential protein expression of KCC2 and NKCC1 in VB and NRT.
- NRT neurons exhibit a more depolarized GABA reversal potential than VB neurons.
- KCC2-mediated Cl− extrusion is present in VB, but not in NRT neurons.
- Inhibiting NKCC1 did not restrain neuronal Cl− uptake in the NRT.
- Figure 1 Differential expression of KCC2 and NKCC1 in VB and NRT detected by immunostaining and Western blot analysis.
- Figure 2 NRT neurons exhibited more positive GABA reversal potentials than VB neurons.
- Discussion
- Figure 3 KCC2-mediated Cl− extrusion is present in VB, but not in NRT neurons.
- Figure 4 Inhibition of NKCC1 did not restrain neuronal Cl− uptake in the NRT.
- Methods
- Slice preparation and immunoblots.
- Electrophysiological recordings and data analysis.
- Immunohistochemistry.
- Cell harvesting, reverse transcription and single-cell RT-PCR.
- Statistical analysis.
- Table 1 Primers used for single cell RT-PCR.
- Acknowledgements