Eichelmann, Ann-Kathrin; Matuszcak, Christiane; Lindner, Kirsten; Haier, Jörg; Hussey, Damian J.; Hummel, Richard: Complex role of miR-130a-3p and miR-148a-3p balance on drug resistance and tumor biology in esophageal squamous cell carcinoma
Inhalt
- Confirmation of successful transfection.
- Both, up- and downregulation of miR-130a-3p and miR-148a-3p increased sensitivity towards chemotherapy in ESCC.
- Both, up- and downregulation of miR-148a-3p inhibited adhesion and migration, and induced apoptosis.
- miR-130a-3p and miR-148a-3p regulate p53-dependent apoptosis-pathway.
- Figure 1 Chemotherapy response in esophageal cancer cell lines is affected by (a) miR-130a-3p and (b) miR-148a-3p.
- Figure 2 Effect of miR-148a-3p expression on biological behaviour in ESCC cell lines: adhesion, migration and apoptosis.
- Discussion
- Figure 3 Western Blot analysis of potential targets of miR-130a-3p (a) and miR-148a-3p (b).
- Figure 4 Analysis of p53-pathway in relation to miRNA expression and potential targets.
- Methods
- Cell culture.
- Transient transfection.
- RNA extraction and qRT-PCR.
- Drug treatment.
- Adhesion and migration assays.
- Apoptosis analysis.
- Western blot.
- Statistical analysis.
- Acknowledgements