Eißing, Anna ; Fischer, Daniel; Rauch, Ilka ; Baumann, Anne; Schebb, Nils Helge ; Karst, Uwe; Rose, Karsten ; Klumpp, Susanne ; Krieglstein, Josef: Acetylcholine content and viability of cholinergic neurons are influenced by the activity of protein histidine phosphatase
Inhalt
- Methods
- Materials
- Monoclonal PHP antibody
- Cell culture and cell extracts
- Immunoblotting
- Transfection of SN56 cells
- Overexpression of PHP
- Downregulation of PHP by siRNA
- Analysis of cell damage
- Acetylcholine measurement
- Statistical analysis
- Results
- Overexpression of PHP and cell damage
- PHP overexpression and acetylcholine level
- PHP downregulation and cell viability
- Acetylcholine level after PHP downregulation
- Discussion
- Acknowledgements
- Author details
- Authors' contributions
- References