Schuch, Ronaldo ; Agelopoulos, Konstantin ; Neumann, Anna ; Brandt, Burkhard; Bürger, Horst Karl Erich ; Korsching, Eberhard Ulrich: Site-specific chromatin immunoprecipitation: a selective method to individually analyze neighboring transcription factor binding sites in vivo
Inhalt
- Background
- Findings
- Results
- Methodological design
- Analysis of the allocation of Sp1 binding sites in EGFR expressing cells
- Application of Sp1-directed site-specific ChIP on EGFR expressing tissue samples
- Discussion
- Conclusions
- Methods
- Site-specific Chromatin immunoprecipitation
- PCR based detection of immunoprecipitated TFBSs
- PCR based controls of site-specific ChIP
- Availability and requirements
- Ethics statement
- Acknowledgements
- Author details
- Authors' contributions
- Competing interests
- References