Rose, Karsten: Interaction of ATP with fibroblast growth factor 2: biochemical characterization and consequence for growth factor stability
Inhalt
- Methods
- Equilibrium-microdialysis
- CD measurement
- Chemical cross-linking experiments
- Degradation of FGF2 and FGF2(K134A) by NE
- Elisa
- Measurement of ATP content
- Results
- Dissociation constant of ATP-binding to FGF2
- Conformational change of secondary structure of FGF2 after ATP-binding
- Influence of ATP on dimerization behaviour of FGF2
- Stability of FGF2 and FGF2 (K134A) against proteolytic digestion
- Stability of FGF2 in aqueous solution is increased after ATP-binding
- Stability of FGF2 depends on number of phosphate residues of used stabilizers
- Discussion and conclusions
- Acknowledgements
- Authors' contributions
- References