Dhayat, Sameer; Träger, Max M.; Rehkämper, Jan; Ströse, Anda Jana; Steinestel, Konrad Ernst; Wardelmann, Eva; Kabar, Iyad; Senninger, Norbert: Clinical Impact of Epithelial-to-Mesenchymal Transition Regulating MicroRNAs in Pancreatic Ductal Adenocarcinoma
Inhalt
- Introduction
- Results
- In-Vitro Expression of EMT-Regulating MicroRNAs and Proteins
- Clinicopathologic Characteristics
- Expression of EMT-Regulating MicroRNAs in Clinical Solid and Liquid Biopsies
- Expression of EMT-Marker Proteins in Human Pancreatic Tissue and Blood Serum Samples
- Diagnostic Impact of EMT-Regulating MicroRNAs
- Potential Prognostic Impact of EMT-Regulating MicroRNAs
- Discussion
- Materials and Methods
- Patients and Samples
- Selection of MicroRNAs and Their Target Proteins
- RNA Isolation and Quantification of EMT-Regulating MicroRNAs
- Tissue Array Immunohistochemistry
- Cell Lines and MicroRNA Transfection
- Western Blotting
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Statistical Analysis
- Limitations of This Study
- Conclusions
- References