Chakraborty, Deblina; Zenker, Stefanie; Rossaint, Jan; Hölscher, Anna; Pohlen, Michéle Yvonne; Pohlen, Michéle; Zarbock, Alexander; Roth, Johannes; Vogl, Thomas: Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner
Inhalt
- Materials
- Expression and Purification of Murine S100A8 Protein
- Isolation of Lung AECs
- Culture of Primary AECs
- Murine Model of Lung Inflammation
- Flow Cytometry
- Immunofluorescent Staining of AECs for TLR4-MD-2 and RAGE
- IL-6 Analysis by ELISA
- S100A8/A9 and IgM Analysis by ELISA
- Cytokine/Chemokine Measurements by Bead Based Immunoassay
- Statistical Analysis
- Results
- Optimizing Isolation, Purification, and Culturing of AECs
- S100A8-Induced IL-6 Release from AECs follows a Dose- and Time-Dependent Pattern
- Cytokine/Chemokine Response Induced by S100A8 in AECs Compared to LPS
- Analysis of TLR4-MD-2 and RAGE Expression in AECs
- S100A8 Induces IL-6 Release in AECI and AECII via TLR4
- Absence of Endogenous S100A8/A9 Hampers Neutrophil Recruitment in ALI Affected Murine Lungs
- Discussion
- Ethics Statement
- Author Contributions
- Acknowledgments
- Funding
- Supplementary Material
- References