López Tobón, Alejandro; Suresh, Megalakshmi; Jin, Jing; Vitriolo, Alessandro; Pietralla, Thorben; Tedford, Kerry; Bossenz, Michael; Mahnken, Kristina; Kiefer, Friedemann; Testa, Giuseppe; Fischer, Klaus-Dieter; Püschel, Andreas W.: The guanine nucleotide exchange factor Arhgef7/βPix promotes axon formation upstream of TC10
Inhalt
- Results
- Arhgef7 is required for axon formation.
- Arhgef7 is required for axon formation in vivo.
- Arhgef7 acts upstream of TC10 during axon specification.
- Figure 2 Arhgef7 is required for axon formation.
- Figure 3 Arhgef7 is required for axon formation in the cortex.
- Figure 4 Active Cdc42 is not able to rescue the loss of Arhgef7.
- Figure 5 Arhgef7 interacts with TC10.
- Discussion
- Figure 6 Active TC10 rescues the loss of axons caused in Arhgef7-deficient neurons.
- Materials and Methods
- Antibodies.
- Plasmids.
- Culture of primary cortical and hippocampal neurons.
- Arhgef7 conditional knockout mouse.
- Immunofluorescence staining of neuronal cultures.
- Biochemistry.
- Molecular Modeling.
- Statistical analysis.
- Data availability statement.
- Acknowledgements