Hamer, Stefanie; Hamer, Stefanie Nicole; Cord-Landwehr, Stefan; Landwehr, Stefan Cord-; Biarnés, Xevi; Planas, Antoni; Waegeman, Hendrik; Moerschbacher, Bruno; Kolkenbrock, Stephan: Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases
Inhalt
- Figure 1 SDS-PAGE and corresponding western blot of recombinant NodB from Rhizobium sp. GRH2 and COD from Vibrio cholerae purified from the crude extract of E. coli BL21 (DE3) [pET-22b(+)::nodB_StrepIIC] and E. coli Rosetta 2 (DE3) [pLysSRARE2, pET-22b(+)::cod_StrepIIC], respectively.
- Table
- Figure 2 Determination of the pattern of acetylation (PA) of GlcNAc5 (a5) after hydrolysis with NodB from Rhizobium sp. GRH2 by enzymatic sequencing in combination with UHPLC-ELSD-ESI-MS analysis.
- Table
- Table
- Figure 3 UHPLC-ELSD-ESI-MS analysis of in vitro combinations of NodB and COD.
- Figure 4 Enzymatic sequencing in combination with UHPLC-ELSD-ESI-MS analysis of double-deacetylated chitosan pentamer (a3d2).
- Figure 5 Structures of AA and DA substrates in the active site of COD.
- Figure 6 Production and UHPLC-ELSD-ESI-MS analysis of doubly deacetylated chitosan tetramers and pentamers at mg-scale.
- References