Schelleckes, Katrin ; Schmitz, Boris ; Lenders, Malte ; Mewes, Mirja ; Brand, Stefan-Martin; Brand, Eva: ZFP226 is a novel artificial transcription factor for selective activation of tumor suppressor KIBRA
Inhalt
- Design of the artificial zinc finger ZFP226.
- Expression of ZFP226 leads to activation of the KIBRA core promoter.
- ZFP226 induces the upregulation of endogenous KIBRA.
- ZFP226 activates YAP and LATS1 phosphorylation.
- ZFP226 induces apoptosis of breast cancer cells.
- Figure 1 ZFP226 target sequence in the human KIBRA promoter, amino acid assembly and cellular localization.
- Figure 2 Target activation of KIBRA promoter P1a by ZFP226.
- Figure 3 ZFP226 overexpression activates KIBRA mRNA and protein expression.
- Figure 4 ZFP226 overexpression enhances YAP and LATS1 phosphorylation.
- Discussion
- Figure 5 ZFP226 induced apoptosis in MCF-7 cells.
- Conclusion
- Methods
- Construction of the ZFP226 transcription factor.
- Cell culture, transfection and reporter gene assay.
- Western blot.
- Immunofluorescence.
- Real-time PCR.
- Annexin V apoptosis assay.
- Statistical analysis.
- Data availability.
- Acknowledgements