Rahmann, Hendrik: Realization of low dose XPCS experiments for the investigation of protein dynamics. 2020
Inhalt
- Contents
- Abstract
- Zusammenfassung
- Introduction
- Theoretical Background of X-ray Scattering Techniques
- Basics of X-ray Scattering
- Small Angle X-ray Scattering (SAXS)
- Coherent X-ray Scattering
- X-ray Photon Correlation Spectroscopy (XPCS)
- Signal-to-noise ratio of XPCS experiments
- Radiation damage
- Protein interactions and phase transitions
- Biomolecular crowding and biocondensates
- Intermolecular interactions in protein solutions
- Thermodynamic fundamentals of phase transitions
- Spinodal decomposition and Cahn-Hilliard equation
- Arrested State and Glass Transition
- IgG-PEG model system
- Experimental setup and sample environment
- PETRA III synchrotron at DESY
- The P10 Coherence Applications Beamline
- Data treatment using Eiger 4M detector
- IgG sample preparation
- IgG sample measurement protocol
- Data Analysis and Discussion
- Raw data preparation
- Radiation damage to IgG proteins
- USAXS analysis of the quenched IgG model system
- SAXS data
- Conclusion and Outlook
- Appendix
- Bibliography
- Acknowledgement