Jung, Niklas: Synthesis of Telechelic Polymers as Functional Building Blocks in Material Science and Biomedical Applications Based on Thermoresponsive Poly(2 Oxazoline)s [...]. 2021
Inhalt
- Content
- 1 Summary
- Zusammenfassung
- 2 Structures and Abbreviations
- 3 Aim and Outline of the Thesis
- 4 Introduction
- 4.1 Thermoresponsive Polymers
- 4.2 Cationic Ring Opening Polymerization (CROP) of 2-Oxazolines
- 4.3 Reversible Addition-Fragmentation Chain Transfer Polymerization (RAFT Polymerization)
- 5 Results and Discussion
- 5.1 Synthesis and Post-functionalization Strategies of Poly(2-alkyl-2-oxazoline)s
- 5.2 Synthesis and (Post-)Functionalization Strategies of N-Substituted Poly(acrylamide)s
- 5.3 Synthesis of thermoresponsive Polymer-DNA Conjugates as Building Blocks for Smart Materials
- 5.4 Hetero-Telechelic Poly(2-oxazoline)s as Linkers for Biosensing Assays
- 5.5 Amino-Functionalized Poly(acrylamide)s as Alternative Safe Non-viral DNA Carriers for Transfection Applications: An In Vitro Study
- 6 Experimental Section
- 6.1 Equipment and Methods
- 6.2 Chemicals
- 6.3 Synthesis of Initiators and Oxazolines for CROP
- 6.4 Synthesis of Acrylamide Monomers and RAFT-Agents
- 6.5 Experimental Procedures for Synthesis of Poly(2-Oxazoline)s
- 6.6 Post-functionalization Reactions of Poly(2-Oxazoline)s
- 6.7 General Procedures for RAFT-Polymerizations
- 6.8 Post-functionalization Reactions of Poly(acrylamide)s
- 6.9 Procedures for UV/Vis and Irradiation Experiments
- 6.10 Experimental Details of Surface Plasmon-Enhanced Fluorescence Spectroscopy (SPFS)
- 6.11 Experimental Details of the Cell and Transfection Experiments
- 7 Appendix
- 7.1 Spectra of Non-Polymeric Compounds
- 7.2 Polymer Spectra
- 7.3 Poly(Acrylamide) Spectra
- 7.4 Additional UV/Vis Spectra
- 7.5 Additional Data Gel Electrophoresis Experiments
- 7.6 Additional Data of the SPFS Measurements
- 7.7 Additional Data of Transfection Experiments
- 8 References
- Acknowledgements