de
en
Close
Detailsuche
Bibliotheken
Projekt
Imprint
Privacy Policy
Search Details
Quicksearch:
OK
Result-List
Title
Title
Content
Content
Page
Page
Search the document
Adeno-associated virus capsid protein expression in Escherichia coli and chemically[...]
Expression and purification of VP3 proteins.
Figure 1 Expression of VP3wt protein in E.
Assembly and characterization of VLPs.
Cellular uptake of VP3wt VLPs.
Effect of AAP2 on in vitro assembly.
Surface modification but not C-terminal extension is compatible with capsid antibody A20 mAb detection.
Figure 2 Characterization of VP3wt VLPs.
Figure 3 Fluorescent microscopy images of internalization.
Figure 4 Impact of AAP2 on in vitro assembly.
Discussion
Figure 5 The effect of incorporation of a small peptide, either into the 587-loop or at the C-terminus of VP3wt, on in vitro assembly.
Methods
Expression and purification of proteins.
SDS-PAGE and western blot.
In vitro assembly of VLPs.
DLS, AFM Characterization of VLPs.
ELISA.
Cellular uptake of VP3wt VLPs.
Acknowledgements
Journal Article
Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly
Place and Date of Creation
2019
JPEG-Download
avaibable widths
JPEG large
JPEG original
show thumbnails
hide thumbs
