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Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small[...]
Materials and Methods
Protein Expression and Purification
Analytical Size Exclusion Chromatography
Cell Motility Assay
Small-Angle X-ray Scattering
Photon Correlation Spectroscopy
Static Light Scattering
Molecular Mass Determination with SLS—The Zimm-Plot
Density, Refractive Index and Refractive Index Increment Measurement
Molecular Mass Calculation from SAXS Data
Prediction and Evaluation of a Possible Structure Model
Results and Discussion
SEC Analysis
SAXS Data Analysis
Calculation of SAXS Data with CRYSOL
Photon Correlation Spectroscopy (PCS) Data
Calculation of Hydrodynamic Data with HYDROPRO
Zimm Analysis
Cell Motility Assay
Conclusions
References
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Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering
Entstehung
2019
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