pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

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Title
pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
Author
Schaffert, Lena ; Jacob, Lucas ; Schneiker-Bekel, Susanne ; Persicke, Marcus ; März, Camilla ; Rückert, Christian ; Pühler, Alfred ; Kalinowski, Jörn
Is part of
Microbiology Resource Announcements, Vol. 9 Issue 39
Published
2020
Language
English
Document type
Journal Article
URN
urn:nbn:de:0070-pub-29461574
DOI
10.1128/mra.00596-20

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<jats:title>ABSTRACT</jats:title>
<jats:p>The pSETT4 vector integrates into the <jats:italic>Actinoplanes</jats:italic> sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other <jats:italic>Actinomycetales</jats:italic> by exchanging the promoter.</jats:p>

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