Windmöller, Beatrice Ariane: Targeting of MYC- and NF-κB-signaling in primary human cancer stem-like cells. 2021
Inhalt
- Introduction
- Results
- Tumor Characterization
- BKZ-2 and BKZ-3 Demonstrate Characteristics of Stemness and EMT–Relation to Immune Response Involved Targets
- BKZ-2 and BKZ-3 Feature Neuroendocrine, Neural Crest and Neuronal Characteristics
- ATRA-Treatment Leads to a Contrary Switch in Growth Habits of BKZ-2 and BKZ-3
- NMYC Copy Number Gain Sensitize BKZ-2 and BKZ-3 for MYC/NMYC Inhibitor Induced Apoptosis
- Discussion
- Materials and Methods
- Patients Clinical Characterisation and Oncological Treatment
- Colorectal Cancer Cell Line Establishment and Cell Culture
- Immunocytochemistry and Immunohistochemistry
- Sphere-Formation and ATRA-Treatments
- Real-Time PCR
- MYC Inhibitor Treatment
- Flow Cytometry
- Statistical Analysis
- Conclusions
- References
- Introduction
- Materials and Methods
- Cancer Stem-like Cell Population Establishment and Cultivation
- Immunocytochemistry
- RNA Isolation and Sequencing
- Preprocessing and Genome Alignment
- Gene Abundance Estimation and Enrichment Analysis
- Results
- Correlative Analysis of Characteristic Markers in Cancer Stem-like Cells from Endometrioid Carcinomas, Glioblastoma Multiforme, Lung- and Prostate Adenocarcinomas
- Global Gene Expression Analysis of Cancer Stem-like Cell Populations Reveals Distinct Clusters
- KEGG Pathway and GO-Term Analysis Reveal Broad Similarities between the Cancer Stem-like Cell Populations
- Discussion
- Conclusions
- References
- Introduction
- Materials and Methods
- Lung Cancer Stem Cell-like Cell Population Establishment and Cell Culture
- Immunocytochemistry
- Western Blot
- Senescence Assay
- Quantitaive Polymerase Chain Reaction
- Inhibitor Treatments
- Statistical Analysis
- Results
- Squamous Cell Carcinoma- and Adenocarcinoma-Derived Cells Depicted Stemness-like Phenotype
- Tumor Necrosis Factor- Stimulation Activated NF-B RelA in Squamous Cell Carcinoma- and Adenocarcinoma-Derived Lung Cancer Stem Cell-like Cells
- Inhibition of MYC and NMYC in Squamous Cell Carcinoma- and Adenocarcinoma-Derived Lung Cancer Stem Cell-like Cells Significantly Impaired Cell Survival
- Inhibition of NF-B Signaling Decreased Survival of Squamous Cell Carcinoma- and Adenocarcinoma-Derived Lung Cancer Stem Cell-like Cells
- Discussion
- References