Hachmeister, Henning: A Multimodal Imaging Platform to enable Hyperspectral Coherent Raman Spectroscopy. 2021
Inhalt
- Introduction
- Theory
- Light-matter interactions
- Spontaneous Raman scattering
- Coherent Raman scattering
- Other scattering effects
- Optical fibers
- Optical setup for coherent Raman imaging
- The laser system
- Optical parametric oscillator
- Acousto-optic modulator
- Spatio-temporal overlap of excitation laser beams
- Control of the galvanometer scanning mirrors
- Detection scheme
- Noise reduction approach
- Confocal Raman scattering microscope
- Experimental Results
- Characterization of the spontaneous Raman scattering microscope
- Intracellular lipid analysis of oleaginous microalgae
- Introduction
- Project outline
- Pre-experiments
- Analyzing the impact of nitrogen starvation on the formation of lipid droplets
- Conclusion
- Following stem cell differentiation with label-free imaging techniques
- Hyperspectral CARS imaging
- Introduction
- Effect of varying pulse length on the CARS signal
- Computational analysis of hyperspectral data sets
- Applying PCA on ITSC and iPSC data sets
- Analyzing the impacts of pollutants on early environmental markers
- Following cell differentiation of alveolar epithelium cells with label-free microscopy
- Compact fiber laser source for coherent Raman scattering
- Conclusion
- Publications
- Acknowledgements
- Statutory Declaration