Le, Dinh To: Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli. 2021
Inhalt
- Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly
- Results
- Figure 1 Expression of VP3wt protein in E.
- Assembly and characterization of VLPs.
- Cellular uptake of VP3wt VLPs.
- Effect of AAP2 on in vitro assembly.
- Surface modification but not C-terminal extension is compatible with capsid antibody A20 mAb detection.
- Figure 2 Characterization of VP3wt VLPs.
- Figure 3 Fluorescent microscopy images of internalization.
- Figure 4 Impact of AAP2 on in vitro assembly.
- Discussion
- Figure 5 The effect of incorporation of a small peptide, either into the 587-loop or at the C-terminus of VP3wt, on in vitro assembly.
- Methods
- Expression and purification of proteins.
- SDS-PAGE and western blot.
- In vitro assembly of VLPs.
- DLS, AFM Characterization of VLPs.
- ELISA.
- Cellular uptake of VP3wt VLPs.
- Acknowledgements
- Introduction
- In Vitro Assembly of Virus-Like Particles (VLPs)
- Application of In Vitro-Assembled VLPs
- Conclusions
- References