Barria, Maria Ines; Alvarez, Raymond A.; Law, Kenneth; Wolfson, Deanna L.; Huser, Thomas; Chen, Benjamin K.: Endocytic Motif on a Biotin-Tagged HIV-1 Env Modulates the Co-Transfer of Env and Gag during Cell-to-Cell Transmission. In: Viruses. Jg.13 H. 9. 2021
Inhalt
- Materials and Methods
- Viral Constructs
- Cells and Cell Culture
- Preparation of Viral Particles and Western Blot Analysis
- Cell-Free Infectivity Assay
- Cell-to-Cell Transfer Assay and VS-Mediated Infection Assay
- Env Labeling
- Fixed Cell Microscopy
- Monitoring Env Transfer by Live Cell Imaging
- Statistics
- Results
- Development of an Infectious Biotin Acceptor Peptide (BAP)-Tagged HIV Envelope Virus, HIV BAP-V4
- Live Cell Labeling of Surface Biotin in HIV BAP-V4-Infected Cells Enables the Measurement of Env Transfer during Cell–Cell Infection
- Visualization of HIV Gag and Env during VS Formation Using a Dual Fluorescent HIV-1 Gag-BAP-V4 Env
- Mutation of a Membrane Proximal Endocytosis Motif Decreases Productive HIV-1 Infection through Cell–Cell Transmission
- Loss of Endosomal Recycling Promotes Discoordinated Transfer of Gag and Env across VSs
- Discussion
- References