Maynard, Daniel; Chibani, Kamel; Schmidtpott, Sonja; Seidel, Thorsten; Sproß, Jens; Viehhauser, Andrea; Dietz, Karl-Josef: Biochemical Characterization of 13-Lipoxygenases of Arabidopsis thaliana. In: International Journal of Molecular Sciences. Jg.22 H. 19. 2021
Inhalt
- Results
- In Silico and Subcellular Targeting of Arabidopsis 13-Lipoxygenases
- Purification and Enzymatic Characteristics of Arabidopsis 13-Lipoxygenases
- Arabidopsis 13-LOX Isoforms Mediate the Synthesis of 12-OPDA and OCPD
- Effect of Metal Ions and LOX-Associated Phytohormones on Arabidopsis 13-LOX Activities
- The Role of Cysteine Residues in Activity Regulation of AtLOX4
- Discussion
- Synthesis of Functional 13-LOX Isoforms
- Subcellular Localization of 13-LOX Isoforms
- Substrate Specificities and Evolutionary Relations
- Differential Impact of Divalent Cations on 13-LOX Isoforms
- Suicide Inhibition and Feedback Regulation
- Cyclopentenone Prostaglandins as Potential LOX Inhibitors
- Materials and Methods
- Data processing, Databases and Computational Studies
- Chemicals and Reagents
- Subcellular Localization Studies
- Cloning, Expression and Preparation of Lipoxygenase Wild-Type and Variant LOX2, LOX3, LOX4, LOX6 and LOX4 C203S
- Lipoxygenase Activity
- Effect of pH and Various Compounds on LOX Activities
- Effect of Divalent Cations on LOX-Catalyzed LeA Dioxygenation
- LOX-Mediated Synthesis of 12-OPDA, DHA-Peroxide, ARA-Peroxide and OCPD
- Determination of Iron Content and Free Cysteines
- Ex Vivo Evaluation of 12-OPDA as a LOX Inhibitor
- Conclusions
- References