Täuber, Sarah ; Blöbaum, Luisa; Wendisch, Volker F. ; Grünberger, Alexander: Growth response and recovery of Corynebacterium glutamicum colonies on single-cell level upon defined pH stress pulses. In: Frontiers in Microbiology. Jg.12. 2021, S. 711893 ff.
Inhalt
- Precultivation, Bacterial Strain, Medium
- Live/Dead Staining
- Chip Preparation
- Chip Design
- Setup and Microfluidic Cultivation
- Live-Cell Imaging
- Image and Data Analyses
- Results
- Corynebacterium glutamicum Growth in Constant pH Environments
- Corynebacterium glutamicum Growth in Single pH Stress Pulse Experiments
- Corynebacterium glutamicum Growth at the Colony Level After Various pH Stress Pulses
- Corynebacterium glutamicum Growth at the Single-Cell Level After Various pH Stress Pulses
- Single-Cell Growth Behavior and Adaptation During the First Post-stress Division
- Discussion
- Corynebacterium glutamicum Growth in Constant pH Environments
- Corynebacterium glutamicum Growth in Single pH Stress Pulse Experiments
- Conclusion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References