Mustafa, Rasem; Rawas, Chahinaz; Mannal, Nadja; Kreiner, Grzegorz; Spittau, Björn; Kaminska, Katarzyna; Yilmaz, Rustem; Potschke, Christina; Kirsch, Joachim; Liss, Birgit; Tucker, Kerry L; Parlato, Rosanna: Targeted Ablation of Primary Cilia in Differentiated Dopaminergic Neurons Reduces Striatal Dopamine and Responsiveness to Metabolic Stress. In: Antioxidants. Jg.10 H. 8. 2021
Inhalt
- Materials and Methods
- Mice
- Brain Dissection and Tissue Preparation for Histology and Western Blot
- Immunohistochemistry
- Analysis of Primary Cilia Frequency and Length and TH and DAT Immunoreactivity
- HPLC Analysis of Dopamine Content
- Western Blot Analysis
- Electrophysiological Multi-Electrode Array (MEA) Recordings
- MPTP Treatment
- Stereological Analysis
- Statistical Analysis
- Results
- Generation of Mutant Mice Lacking Primary Cilia in Differentiated DA Neurons
- Loss of Primary Cilia in Differentiated DA Neurons Reduces Nigrostriatal DA Projections and Striatal Dopamine Content
- Loss of Primary Cilia in Differentiated DA Neurons Reduces the Number of Dopamine-Excited SN Neurons
- Loss of Primary Cilia in Differentiated DA Neurons of Ift88 cKO Mutants Renders Them Insensitive to the Neurotoxin MPTP
- Loss of Primary Cilia in DA Neurons Results in Increased Primary Cilia Length in the Striatum of Ift88 Conditional Knock-Out Mice
- Discussion
- References