Faßhauer, Patrick; Busche, Tobias; Kalinowski, Jörn; Mäder, Ulrike; Poehlein, Anja; Daniel, Rolf; Stülke, Jörg: Functional Redundancy and Specialization of the Conserved Cold Shock Proteins in Bacillus subtilis. In: Microorganisms. Jg.9 H. 7. 2021
Inhalt
- Materials and Methods
- Bacterial Strains, Growth Conditions, and Phenotypic Characterization
- DNA Manipulation and Genome Sequencing
- Construction of Translational LacZ Reporter Gene Fusions
- Construction of Mutants
- RNA-Sequencing
- Qualitative PCR and Real-Time Quantitative Reverse Transcription PCR
- Microscopy
- Results
- Relative Contribution of the Cold Shock Proteins to the Growth of B. subtilis
- Suppressor Analysis of the CspB CspD Double Mutant
- A Modified CspC Protein Can Take over the Functions of CspB and CspD
- RNA-Seq Analysis Suggests a Role for Cold Shock Proteins in Transcription Termination and Elongation
- Analysis of Csp-Dependent Transcription Read-Through
- Discussion
- References