Sargsyan, Yelena; Bickmeyer, Uta; Gibhardt, Christine S; Streckfuss-Bomeke, Katrin; Bogeski, Ivan; Thoms, Sven: Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells. In: Life science alliance. Jg.4 H. 9. 2021
Inhalt
- Development and validation of Ca2+ sensors for peroxisomal Ca2+
- Peroxisomal Ca2+ in non-excitable cells largely follows cytosolic Ca2+
- Peroxisomal Ca2+ is not regulated by the mitochondrial calcium uniporter (MCU) complex
- Peroxisomal Ca2+ in cardiomyocytes rises with cytosolic Ca2+ increase
- Peroxisomal Ca2+ oscillates in cardiomyocytes
- Discussion
- Materials and Methods
- DNA constructs
- Cells, cell culture, and immunofluorescence
- siRNA-mediated protein knockdown and qPCR
- RT-qPCR
- Ca2+ measurements
- pH control experiments
- Statistical analysis
- Supplementary Information
- Acknowledgements
- Author Contributions
- Conflict of Interest Statement
- Bootman MD, Berridge MJ, Roderick HL (2002) Calcium signalling: More messengers, more channels, more complexity. Curr Biol ...