Witte, Kaya; Pfitzenmaier, Jesco; Storm, Jonathan; Lütkemeyer, Melanie; Wimmer, Clara; Schulten, Wiebke; Czaniera, Nele; Geisler, Marvin; Förster, Christine; Wilkens, Ludwig; Knabbe, Cornelius; Mertzlufft, Fritz; Kaltschmidt, Barbara; am Esch, Jan Schulte; Kaltschmidt, Christian: Analysis of Several Pathways for Efficient Killing of Prostate Cancer Stem Cells: A Central Role of NF-κB RELA. In: International Journal of Molecular Sciences. Jg.22 H. 16. 2021
Inhalt
- Introduction
- Results
- Primary Isolated Prostate Cancer Cells Exhibit Stemness Characteristics
- Prostate Cancer Stem Cells Display High Levels of Epithelial-to-Mesenchymal Transition-Associated Genes and Distinct Expressions of Programmed Cell Death Ligands
- Programmed Cell Death 1 Inhibitor Pembrolizumab Caused No Enhancement of Low-Level Natural Killer Cell-Mediated Lysis on Primary Prostate Cancer Stem Cells
- Interferon Gamma Treatment of Prostate Cancer Stem Cells Revealed High-Level Upregulation of Major Histocompatibility Complex Class I with Only Moderate Cytotoxic Effects
- Tumor Necrosis Factor-Induced Prostate Cancer Stem Cell Killing Is Caused by Blunted NF-B Activation
- Discussion
- Materials and Methods
- Patients Clinical Characterizations
- Isolation and Cultivation of Primary Prostate Cancer Stem Cell Populations
- Culture of Free-Floating Spheres
- Immunochemistry
- RNA Isolation and Reverse-Transcription PCR
- Examination of the Population Doubling Time
- Flow Cytometry
- Co-Culture with Natural Killer Cells
- Stimulation of the Major Histocompatibility Complex Class I
- NF-B Transcription Factor Activation
- Statistical Analysis and Figure Design
- References