Budde, Dana; Albano, Gian-Luca; Noll, Thomas; Jurkiewicz, Elke: Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation
Inhalt
- 2 EXPERIMENTAL
- 2.1 Cell cultivation
- 2.2 Preparation of LMC mixture
- 2.3 Cell growth and flow cytometry analysis
- 2.4 IgG quantification
- 3 METHODS
- 3.1 Dose-response and spiking experiments
- 3.2 Interaction study
- 3.3 Fed-batch cultivation
- 3.4 Flow cytometry analysis
- 3.5 Isobologram analysis
- 4 RESULTS AND DISCUSSION
- 4.1 Growth inhibiting effect of the LMC-mix
- 4.2 Productivity of LMC-treated cultures
- 4.3 Interactions of LMCs
- 5 CONCLUSION
- ACKNOWLEDGMENTS
- AUTHOR CONTRIBUTIONS
- CONFLICT OF INTEREST
- REFERENCES