Conrad, Karen S.; Cheng, Ting-Wen; Ysselstein, Daniel; Heybrock, Saskia; Hoth, Lise R.; Chrunyk, Boris A.; am Ende, Christopher W.; Krainc, Dimitri; Schwake, Michael ; Saftig, Paul; Liu, Shenping; Qiu, Xiayang; Ehlers, Michael D.: Lysosomal integral membrane protein-2 as a phospholipid receptor revealed by biophysical and cellular studies. In: Nature Communications. Jg.8 H. 1. 2017
Inhalt
- Structure determination of a lipid-bound LIMP-2 dimer
- Ligand binding sites and dimerization interface
- LIMP-2 dimer binds PS liposomes but not GCase
- Detection of LIMP-2 dimer in cells
- LIMP-2 mediates cellular uptake of PS liposomes
- Discussion
- Methods
- Protein expression and purification
- Crystallization
- Data collection and structure determination
- Liquid chromatography–nobreakmass spectrometry
- Liposome generation
- SPR
- Multi-angle static light scattering
- DLS
- Negative stain EM
- Cross-linking experiments
- Electrophoresis and immunoblotting
- Bimolecular fluorescence complementation
- Cell culture and immunocytochemistry
- Time-lapse imaging
- Phospholipid uptake assay
- Synthesis of MDW941
- GCase activity assays
- Data availability
- References
- Acknowledgements
- Author contributions
- Competing interests
- ACKNOWLEDGEMENTS