Krahn, Irene; Bonder, Daniel; Torregrosa, Lucia; Stoppel, Dominik; Krause, Jens; Rosenfeldt, Natalie; Meiswinkel, Tobias; Seibold, Gerd M.; Wendisch, Volker F.; Lindner, Steffen: Evolving a new efficient mode of fructose utilization for improved bioproduction in Corynebacterium glutamicum. In: Frontiers in Biotechnology and Bioengineering. Jg.9. 2021
Inhalt
- Materials and Methods
- Strains and Plasmids Used
- Culture Conditions and Growth Experiments
- Analysis of Sugars and Organic Acid Concentration, and Amino Acid Production
- 13C Isotopic Labeling of Proteinogenic Amino Acids
- Sugar Uptake Measurements
- Results
- Construction and Characterization of Strains Lacking Fructose-Specific PTS and 1-Phosphofructokinase Genes
- Adaptive Evolution for Growth on Fructose
- Evidence for Generation of Fructose 6-Phosphate From Fructose via Glucose-Specific PTS
- Complementation of ptsG ptsF by ptsG Overexpression
- Faster Fructose Uptake Mediated by the Selected PtsG Variants
- 13C-Labeling Experiments Reveal Substantially Higher oxPPP Flux
- Evolved and Reverse-Engineered Strains Showed Reduced Overflow Metabolism
- PtsG Catalyzed Fructose Utilization Improved l-Lysine Production
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References