Hess, Natalia; Richter, Simon; Liebthal, Michael; Dietz, Karl-Josef; Mustroph, Angelika: The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. In: Antioxidants. Jg.10 H. 3. 2021
Inhalt
- Materials and Methods
- Plant Material, Growth Conditions and Treatment
- Creation of CRISPR/Cas9 Knockout Lines for AtPFK5
- Cultivation of Trypanosoma Brucei Brucei
- Agrobacterium tumefaciens-Mediated Transient Gene Expression in Nicotiana benthamiana Plants
- Cloning and Site-Directed Mutagenesis of PFKs for Transient Overexpression in Nicotiana benthamiana
- Preparation of Chloroplast Extracts and Treatment
- Enzyme Analysis
- Sequence Alignments and Statistics
- Results
- Arabidopsis Contains Six Active PFK Isoforms
- Arabidopsis PFKs Are Sensitive to Redox Modifications
- PFK Isoforms Contain Multiple Conserved Cys
- Mutations of Conserved Cys Modify Enzyme Activity
- AtPFK5 Is a Target of Thioredoxin-f1
- A Knockout of AtPFK5 Has Little Effects in Arabidopsis Plants
- Discussion
- Redox Sensitivity of Plant and Trypanosoma PFKs
- Plastidic PFKs Are Redox Regulated through the CXDXXC Motif
- What Could Be the Function of Redox-Regulated AtPFK5?
- Conclusions
- References