Haupka, Carsten ; Fernandes de Brito, Luciana; Busche, Tobias; Wibberg, Daniel; Wendisch, Volker F.: Genomic and transcriptomic investigation of the physiological response of the methylotroph Bacillus methanolicus to 5-aminovalerate. In: Frontiers in Microbiology. Jg.12. 2021
Inhalt
- Materials and Methods
- Microorganisms and Cultivation Conditions
- Recombinant DNA Work
- Transcriptomics: Cultivation and RNA Isolation
- Transcriptomics: Preparation of cDNA and Differential RNA-Seq
- ALE
- Multi-Tolerance Analysis
- Whole-Genome Sequencing
- Analysis of Proteins With Non-synonymous Amino Acid Exchanges
- High-Performance Liquid Chromatography (HPLC)
- Results
- 5AVA Impaired Growth at Low Concentrations
- Differential RNA-Seq Analysis Revealed Transcriptomic Response to 5AVA
- ALE Overcame 5AVA Toxicity
- ALE Strain AVA10 Showed Tolerance Specific for 5AVA, Not for Related Compounds
- Whole-Genome Sequencing of Mutants and Overexpression Analysis
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References