Luong, Truc Thanh; Nguyen, Minh Tan; Chen, Yi-Wei; Chang, Chungyu; Lee, Ju Huck; Wittchen, Manuel; Ton-That, HyLam; Cruz, Melissa; Garsin, Danielle A.; Das, Asis; Tauch, Andreas; Ton-That, Hung: Ribonuclease J-Mediated mRNA Turnover Modulates Cell Shape, Metabolism and Virulence in Corynebacterium diphtheriae. In: Microorganisms. Jg.9 H. 2. 2021
Inhalt
- Introduction
- Materials and Methods
- Bacterial Strains, Plasmids, Media, and Cell Growth
- Generation of Gene Deletion Mutants in C. diphtheriae
- Recombinant Plasmids
- Protein Purification
- RT-FeDEx Assay
- RNA-Seq Analysis
- Reverse Transcription Polymerase Chain Reactions
- Detection of Diphtheria Toxin
- Electron Microscopy
- Chrome Azurol S (CAS) Assay
- Caenorhabditis Elegans Killing Assay
- Statistical Analysis
- Results
- C. Diphtheriae Encodes Ribonuclease J
- Genetic Disruption of rnj Alters Cell Growth and Morphology
- Transcriptome Analysis of the rnj Mutant
- Reduction of Secreted Diphtheria Toxin in the rnJ Mutant
- Virulence Attenuation of the rnj Mutant in a Caenorhabditis Elegans Model of Infection
- Discussion
- References