Blifernez-Klassen, Olga; Klassen, Viktor; Wibberg, Daniel; Cebeci, Enis; Henke, Christian; Rückert, Christian; Chaudhari, Swapnil; Rupp, Oliver; Blom, Jochen; Winkler, Anika; Al-Dilaimi, Arwa; Goesmann, Alexander; Sczyrba, Alexander; Kalinowski, Jörn; Bräutigam, Andrea; Kruse, Olaf: Phytoplankton consortia as a blueprint for mutually beneficial eukaryote-bacteria ecosystems based on the biocoenosis of Botryococcus consortia. In: Scientific Reports. Jg.11 H. 1. 2021
Inhalt
- Metagenomic survey of the Botryococcus braunii consortia.
- Effect of the bacterial community on the B. braunii host.
- The core community of Botryococcus phycosphere belongs to a niche of biotin auxotrophs.
- Bacterial biotin-auxotrophic symbionts defend their eukaryotic host.
- Discussion
- Materials and methods
- Strains, cultivation conditions and growth determination parameters.
- Hydrocarbon extraction and analysis.
- DNA sample collection and preparation.
- High-throughput 16S rDNA amplicon sequencing.
- (Meta)Genome sequencing, assembly, binning and annotation.
- Gene prediction, taxonomic assignment and functional characterization.
- Phylogenetic analyses.
- References
- Acknowledgements