Kong, Cihang; Bobe, Stefanie; Pilger, Christian; Lachetta, Mario; Øie, Cristina Ionica; Kirschnick, Nils; Mönkemöller, Viola; Hübner, Wolfgang; Förster, Christine; Schüttpelz, Mark; Kiefer, Friedemann; Huser, Thomas; Schulte am Esch, Jan: Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies. In: Frontiers in Physiology. Jg.12. 2021
Inhalt
- Materials and Methods
- Harvesting of Human Liver Biopsy Samples
- Whole Mount Staining of Human Liver
- Preparation of Liver Sections for Non-linear Optical Imaging
- Isolation and Staining of Human Liver Sinusoidal Endothelial Cells (LSEC)
- Optical Projection Tomography
- Light Sheet Fluorescence Microscopy (LSFM)
- Non-linear Optical Microscopy (SRS, CARS, SHG)
- Confocal Microscopy
- Super-Resolution Structured Illumination Microscopy (SR-SIM)
- Results and Discussion
- Mesoscopic Imaging of the Human Liver by Fluorescence Microscopy
- Microscopic Imaging of the Human Liver by Fluorescence and Label-Free Confocal Optical Microscopy
- Super-Resolution Structured Illumination Microscopy of Human Liver Sinusoidal Endothelial Cells
- Conclusion
- Data Availability Statement
- Ethics Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References