Castellani, Lucas G.; Luchetti, Abril; Nilsson, Juliet F.; Pérez-Giménez, Julieta; Wegener, Caren; Schlüter, Andreas; Pühler, Alfred; Lagares, Antonio; Brom, Susana; Pistorio, Mariano; Niehaus, Karsten; Torres Tejerizo, Gonzalo A.: Exopolysaccharide Characterization of Rhizobium favelukesii LPU83 and Its Role in the Symbiosis With Alfalfa. In: Frontiers in Plant Science. Vol.12. 2021
Content
Bacterial Strains and Plasmids
EPS and Total Proteins Quantification
EPS Composition and Structure
Bacterial Matings
DNA Manipulation and Genetic Constructs
Bioinformatics Approaches and Phylogenetic Analyses
Plant Assays
Results and Discussion
Localization and Gene Arrangement of Exopolysaccharide-Related Genes in R. favelukesii LPU83
Capsular Polysaccharide
Galactoglucan
Succinoglycan
Arabinose-Containing Polysaccharide
Cyclic -Glucans (CG) and Mixed-Linkage -Glucans (MLG)
Phylogenetic Analyses of EPS I Genes in Rhizobium favelukesii LPU83
The Exopolysaccharide Produced by R. favelukesii LPU83 Is Similar to the Succinoglycan of E. meliloti
Genomic Regions Required for EPS Production of LPU83
Symbiotic Phenotype of the LPU83 EPS Mutants
Concluding Remarks
Data Availability Statement
Author Contributions
Funding
Acknowledgments
Supplementary Material
References