Wolf, Jacqueline; Koblitz, Julia; Albersmeier, Andreas; Kalinowski, Jörn; Siebers, Bettina; Schomburg, Dietmar; Neumann-Schaal, Meina: Utilization of Phenol as Carbon Source by the Thermoacidophilic Archaeon Saccharolobus solfataricus P2 Is Limited by Oxygen Supply and the Cellular Stress [...]. In: Frontiers in Microbiology. Jg.11. 2021
Inhalt
- Strain and Growth Conditions
- Extraction of Intra- and Extracellular Metabolites and Coenzyme A Derivatives
- GC-MS Measurement of Intra- and Extracellular Metabolites and HPLC-MS Measurement of CoA Derivatives
- Data Processing and Statistical Analysis
- RNA Isolation, Sequencing, and Data Analysis
- Metabolic Modeling
- Results
- Phenotypic Characterization of S. solfataricus P2 Growing on Phenol as Sole Carbon Source
- The Metabolic Response of S. solfataricus P2 to Phenol
- Global Transcriptomic Changes of S. solfataricus P2 During Growth on Phenol
- Discussion
- Global Response of Saccharolobus solfataricus P2 to Phenol and Metabolic Modeling
- Reconstruction of Phenol Degradation in S. solfataricus P2
- Phenol Induces Multiple Stress Responses in S. solfataricus P2
- Conclusion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References