Dreyer, Anna ; Treffon, Patrick; Basiry, Daniel; Jozefowicz, Anna Maria; Matros, Andrea; Mock, Hans-Peter; Dietz, Karl-Josef: Function and Regulation of Chloroplast Peroxiredoxin IIE. In: Antioxidants. Vol.10 Issue 2. 2021
Content
- Materials and Methods
- Plant Material and Growth Conditions
- Cloning
- Heterologous Expression and Purification
- Xylenol Orange Assay
- NADPH-Dependent Peroxidase Activity Measurement
- Hyperoxidation of PRX-IIE
- S-Glutathionylation
- 2-Dimensional SDS-PAGE
- Subcellular Localisation of PRX-IIE
- Affinitiy Chromatography and Mass Spectrometry
- Far Western Blot
- Structural Modeling
- Statistical Analysis
- Results
- PRX-IIE Is Localized to the Chloroplast Stroma
- PRXIIE Detoxifies H2O2 Using the GRX System for Regeneration
- CuOOH-and H2O2-Dependent Hyperoxidation
- S-Glutathionylation of PRX-IIE Occurs at Cysp
- Identification of PRX-IIE Interaction Partners
- Discussion
- Regeneration of Reduced PRX-IIE Limits Catalytic Turnover
- Bulky Substrates Favour Hyperoxidation and Inhibition of PRX-IIE
- Besides Hyperoxidation, Cys121 of PRX-IIE Is Subject to Multiple Posttranslational Modifications
- PRX-IIE Binds to Target Proteins
- 14-3-3 Proteins as Binding Partner of PRX-IIE Open up New Perspectives
- Hypothetical Outlook and Where to Go
- Conclusions
- References