Bakonyi, Daniel; Toelzer, Christine; Stricker, Michael; Hummel, Werner; Niefind, Karsten; Gröger, Harald: Expanding the Application Range of Microbial Oxidoreductases by an Alcohol Dehydrogenase from Comamonas testosteroni with a Broad Substrate Spectrum and [...]. In: Catalysts. Jg.10 H. 11. 2020
Inhalt
- Results and Discussion
- Identification of the Alcohol Dehydrogenase Gene
- Cloning and Heterologous Overexpression of CtADH
- Substrate Specificity, Kinetic Parameters and Enantioselectivity of CtADH
- pH-Profile of Catalytic Activity and Effect of Organic Solvents on CtADH Stability
- Structural Characterization of CtADH
- Materials and Methods
- Origin of Chemicals
- Identification, Cloning and Heterologous Expression of CtADH and SyADH
- Purification of CtADH and SyADH
- Crystallization and Structure Determination of CtADH
- Enzyme Activity Assays
- Effect of Organic Solvents and pH on the Stability and Activity of CtADH
- Determination of the Enantioselectivity
- Conclusions
- References