Braun, Robert; Schönberger, Nora; Vinke, Svenja; Lederer, Franziska; Kalinowski, Jörn; Pollmann, Katrin: Application of Next Generation Sequencing (NGS) in Phage Displayed Peptide Selection to Support the Identification of Arsenic-Binding Motifs. In: Viruses. Jg.12 H. 12. 2020
Inhalt
- Materials and Methods
- Media and Buffer
- Phage Library
- Biopanning
- Experimental Setup
- Column Handling and Target Immobilization
- Phage Library Application and Enrichment
- Sanger Sequencing
- Illumina Sequencing
- Bioinformatic Processing
- Results
- Biopanning Experiments
- Illumina (NGS) Sequencing
- NGS Fraction Composition
- Amino Acid Composition
- Sequence Logo Calculation
- Core Fraction Calculation
- Sequence Motif Calculation and Comparison
- QxQ Motif
- Motif Comparison with 48 h Discovery Database
- Discussion
- Comparison of Sanger Sequencing with Next Generation Sequencing
- NGS: Amino Acid Composition
- NGS: Core Fraction Calculation
- NGS: Motif Enrichment
- QxQ Motif: Metal- and Oxyanion Interaction
- QxQ Motif: Biological Occurrence
- Conclusions
- References