Walter, Tatjana; Veldmann, Kareen; Götker, Susanne; Busche, Tobias; Rückert, Christian; Kashkooli, Arman Beyraghdar; Paulus, Jannik; Cankar, Katarina; Wendisch, Volker F.: Physiological response of Corynebacterium glutamicum to indole. In: Microorganisms. Jg.8 H. 12. 2020
Inhalt
- Materials and Methods
- Bacterial Strains and Molecular Genetic Techniques
- Culture Conditions
- Sequencing of Transcriptomis and Genomic Data
- RNA Isolation, qRT-PCR, Preparation of cDNA Libraries for Sequencing and DeSeq Analysis
- gDNA Isolation, Library Preparation and Sequencing
- Quantification of Amino Acids and Organic Acids by HPLC or GC–MS
- Protein Purification and Electrophoretic Mobility Shift Assay
- Iron Reduction Assay
- Pseudo-Dipeptid Synthesis
- Results
- Growth and Global Gene Expression Response of C. glutamicum to Extracellularly Added Indole
- Transtriptome Analysis of C. glutamicum C1* in Response to Indole-Alanine Dipeptide
- Adaptive Laboratory Evolution for Increasing Indole Tolerance
- Investigation of the Transcriptional Regulator Cg3388
- Discussion
- References