Graf, Michaela; Haas, Thorsten; Teleki, Attila; Feith, Andre; Cerff, Martin; Wiechert, Wolfgang; Nöh, Katharina; Busche, Tobias; Kalinowski, Jörn; Takors, Ralf: Revisiting the Growth Modulon of Corynebacterium glutamicum Under Glucose Limited Chemostat Conditions.. In: Frontiers in Bioengineering and Biotechnology. Jg.8. 2020
Inhalt
- Materials and Methods
- Bacterial Strain and Pre-culture Cultivation
- Continuous Bioreactor Cultivations
- Transcriptome Analysis
- 13C-Metabolic Flux Analysis: Modeling and Computational Procedures
- Metabolic Network Model for 13C-MFA
- A priori Tracer Design
- External Rate Estimation
- Intracellular Flux Estimation
- Determination of Kinetic Parameters
- Results
- Chemostat Cultivations at μ = 0.2, 0.3, and 0.4 h-1
- Metabolomics
- 13C-Labeling Experiment at μ = 0.4 h-1
- Discussion
- Kinetic Studies
- Metabolic and Transcriptional Regulation of Central Metabolism in Chemostat
- Comparability of Chemostat and Batch Observations
- Conclusion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References