Kreienbaum, Maximilian; Dörrich, Anja K.; Brandt, David ; Schmid, Nicole E.; Leonhard, Tabea; Hager, Fabian; Brenzinger, Susanne; Hahn, Julia; Glatter, Timo; Ruwe, Matthias; Briegel, Ariane; Kalinowski, Jörn; Thormann, Kai M.: Isolation and Characterization of Shewanella Phage Thanatos Infecting and Lysing Shewanella oneidensis and Promoting Nascent Biofilm Formation. In: Frontiers in Microbiology. Jg.11. 2020
Inhalt
- Materials and Methods
- Bacterial Strains, Growth Conditions, and Strain Constructions
- Isolation of S. oneidensis Phages Thanatos-1 and -2 and Virion Enrichment
- Phage Particle Enrichment and DNA Isolation
- Chromatographic Purification of Phage Particles
- Genome Sequencing, Assembly and Annotation
- Liquid Chromatography-Mass Spectrometry Analysis on PEG-Precipitated Samples
- LC-MS/MS Analysis of Purified Phage Lysate
- Cryo Electron Microscopy
- Phage Spot Assays
- Lysis Assay During Planktonic Growth
- Temperature and pH Sensitivity Assays
- Latent Period and Phage Burst Size
- Light Microscopy of Infected Shewanella Cells
- Transposon Mutagenesis
- Phage Adsorption Assay
- Static Biofilm Assays
- Static Biofilm Staining and Fluorescent Imaging
- Accession Numbers
- Results
- Isolation of Novel Phages Infecting and Lysing S. oneidensis
- Classification of the Novel Shewanella Phages
- Propagation and Stability of Shewanella Phage Thanatos
- Thanatos Host Recognition Requires the waaC Gene of S. oneidensis
- Presence of Thanatos Differentially Affects S. oneidensis Biofilms
- Discussion
- Data Availability Statement
- Author Contributions
- Funding
- Acknowledgments
- Supplementary Material
- References