Schulte am Esch, Jan; Windmöller, Beatrice Ariane; Hanewinkel, Johannes; Storm, Jonathan; Förster, Christine; Wilkens, Ludwig; Krüger, Martin; Kaltschmidt, Barbara; Kaltschmidt, Christian: Isolation and Characterization of Two Novel Colorectal Cancer Cell Lines, Containing a Subpopulation with Potential Stem-Like Properties: Treatment Options [...]. In: Cancers. Jg.12 H. 9. 2020
Inhalt
- Introduction
- Results
- Tumor Characterization
- BKZ-2 and BKZ-3 Demonstrate Characteristics of Stemness and EMT–Relation to Immune Response Involved Targets
- BKZ-2 and BKZ-3 Feature Neuroendocrine, Neural Crest and Neuronal Characteristics
- ATRA-Treatment Leads to a Contrary Switch in Growth Habits of BKZ-2 and BKZ-3
- NMYC Copy Number Gain Sensitize BKZ-2 and BKZ-3 for MYC/NMYC Inhibitor Induced Apoptosis
- Discussion
- Materials and Methods
- Patients Clinical Characterisation and Oncological Treatment
- Colorectal Cancer Cell Line Establishment and Cell Culture
- Immunocytochemistry and Immunohistochemistry
- Sphere-Formation and ATRA-Treatments
- Real-Time PCR
- MYC Inhibitor Treatment
- Flow Cytometry
- Statistical Analysis
- Conclusions
- References