Böttcher, Michael: Single-cell transcriptome analyses on developmental transitions in mouse pluripotent stem cells. 2020
Inhalt
- Abstract
- Acknowledgments
- List of Figures
- List of Tables
- Abbreviations
- Introduction
- Mouse stem cells – naïve and primed state
- Single-cell transcriptomics
- Applications of scrnaseq in biological research
- Other single-cell -omics technologies
- Transcriptome reference annotation
- Data management
- Dimension reduction and clustering methods
- Random allelic expression
- Materials and Methods
- Cell Culture
- Single-cell capture, imaging, rt and cdna synthesis
- Library preparation and sequencing
- Raw sequencing data processing
- Expression data analysis
- Results
- Transcriptome analysis of the naïve-to-primed transition process
- Characterization of scrnaseq tsne clusters based on gene expression patterns
- c1cage data analysis
- Discussion
- Discovery of two distinct transition states during naïve-to-primed conversion
- Outlook
- Concluding remarks
- References
- Appendix