Henke, Nadja Alina; Austermeier, Sophie; Grothaus, Isabell L.; Götker, Susanne; Persicke, Marcus; Peters-Wendisch, Petra; Wendisch, Volker F.: Corynebacterium glutamicum CrtR and its orthologs in actinobacteria: conserved function and application as genetically encoded biosensor for detection [...]. In: International Journal of Molecular Sciences. Jg.21 H. 15. 2020
Inhalt
- Results
- CrtR Orthologs from Actinobacteria Showed Binding to Their Own Promoters and Derepression by GGPP
- CrtR from C. glutamicum Binds to Heterologous crtR Promoter DNA Sequences
- CrtR Orthologs from Actinobacteria Affected Carotenogenesis and Expression of a CrtE Transcriptional Fusion in C. glutamicum In Vivo
- Construction and Analysis of a GGPP Biosensor
- Discussion
- Materials and Methods
- Bacterial Strains, Media and Growth Conditions
- Recombinant DNA Work and Gene Expression
- Extraction of Carotenoids from Bacterial Cells and HPLC Analysis
- Analysis of Fluorescence via Flow Cytometry
- Overproduction and Purification of the Transcriptional Regulator CrtR
- Electrophoretic Mobility Shift Assay (EMSA)
- Extraction of GGPP and LC-MS Analysis
- References